Table 4. Concentrations of substrates and products of PFK in various mammalian tissues and trypanosomatid glycosomes.

ΔG calculated from eqn (2) using RT = 0.543 kcal mol⁻¹, where R is the gas constant (1.987 cal mol⁻¹K⁻¹) and T = temperature (293 K). Equilibrium constant K is calculated from K = exp (−ΔG/RT). References for concentrations (directly measured in the non-italicised text, derived from models based on whole cell lysate data in italicised text).

	Erythrocyte	Muscle	Brain	T. brucei glycosome
ATP (µM)	1850 [24]	4990 [25]	3325 [30]	3870 [32]
ADP (µM)	180 [24]	600 [29]	309 [30]	1315 [32]
F6P (µM)	15.7 [24]	110 [26]	27 [27]	2400 [31]
F16BP (µM)	7 [24]	32 [28]	200 [27]	1900 [31]
Q (derived)	0.04	0.03	0.69	0.27
ΔG (kcal/mol)	−5.09	−5.21	−3.6	−4.7