Figure 3.

Reversibility in the system and absence of phosphodiester linkage isomerization. UV thermal melting curves of duplex 5 at pH 3.6 (1 M Na⁺, 10 mM citrate buffer) and after adjusting the pH to 6.8 (1 M Na⁺, 10 mM citrate buffer, 200 mM Hepes buffer) and comparison with a control experiment at pH 6.8 (1 M Na⁺, 10 mM citrate buffer, 200 mM Hepes buffer). α = 1 for double-stranded RNA, and α = 0 for single-stranded RNA.