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. 2018 Nov 1;57(45):6382–6386. doi: 10.1021/acs.biochem.8b01080

Table 1. Tm and ΔTm Values of RNA Duplexesa.

duplex GC content (%) Tm (°C) at pH 3.6 Tm (°C) at pH 7.1 ΔTm (°C)
1 53.8 35.5 ± 0.5 65.7 ± 0.3 30.2
2 30.8 17.0 ± 0.0 50.3 ± 0.3 33.3
3 69.2 45.8 ± 0.3 75.3 ± 0.3 29.5
4 50.0 56.5 ± 0.5 84.2 ± 0.3 27.7
a

Values determined by UV thermal melting experiments (±standard deviation). Conditions: 100 mM Na+, 10 mM citrate buffer, pH 3.6 or 7.1.