Table 2. BLI Data for Binding of Thiourea and Adipic Acid Conjugates to 2G12.

Conjugate	Concentration range (μM)	Measurementsa	Experiments	kon ± SEMb (M–1 s–1)	koff ± SEMb (s–1)	KD ± SEMb (nM)
13	0.23–14.4	18	4	5.1 ± 0.3 × 102	8.5 ± 0.8 × 10–5	183 ± 30
14	0.45–7.2	15	3	3.8 ± 0.5 × 103	1.1 ± 0.2 × 10–4	34 ± 4
15a	0.23–14.4	23	4	4.5 ± 1.0 × 103	4.0 ± 1.6 × 10–4	157 ± 61
15c	0.84–6.7	12	3	3.2 ± 0.1 × 104	5.8 ± 0.8 × 10–4	19 ± 3
16c	0.22–13.9	26	4	1.1 ± 0.1 × 104	1.2 ± 0.2 × 10–4	23 ± 6
17	0.22–14.2	26	4	1.0 ± 0.1 × 104	1.2 ± 0.1 × 10–4	19 ± 3
18	0.45–7.1	16	4	4.5 ± 0.7 × 103	1.4 ± 0.2 × 10–3	394 ± 125
20c	0.84–6.8	12	3	2.6 ± 0.2 × 104	1.4 ± 0.1 × 10–3	71 ± 2
20d	0.81–6.5	12	3	1.5 ± 0.1 × 104	2.1 ± 0.4 × 10–3	319 ± 112
21b	0.85–6.8	9	3	1.3 ± 0.1 × 104	7.4 ± 1.1 × 10–4	76 ± 17
21c	0.81–6.5	5	2	3.2 ± 0.1 × 104	2.9 ± 0.1 × 10–4	10 ± 1
32	0.45–7.2	10	3	1.5 ± 0.1 × 104	6.1 ± 0.3 × 10–4	46 ± 4
33	0.46–7.3	2	2	7.3 ± 6.8 × 103	2.0 ± 1.1 × 10–3	274c
34	0.43–6.8	3	2	7.6 ± 4.3 × 104	1.6 ± 0.6 × 10–3	23 ± 4
35	0.45–7.2	2	2	1.9 ± 0.4 × 104	2.3 ± 1.3 × 10–3	121c

^aOnly measurements with R² ≥ 0.85 were included.

^bSEM, standard error of the mean.

^cCalculated as k_off/k_on.