Skip to main content
. 2019 Jan 1;222(1):jeb191106. doi: 10.1242/jeb.191106

Fig. 7.

Fig. 7.

Expression of a UAS-cacophony transgene lacking exon 7 failed to rescue larval crawling and resulted in low cacophony protein expression. (A) Immunoblots using anti-GFP antisera show reduced levels of cacophony-GFP in flies expressing a UAS-cacophony transgene lacking exon 7 [elav>cac(exon7−)::GFP] compared with flies expressing a UAS-cacophony transgene containing exon 7 [elav>cac(exon7+)::GFP] or flies expressing the original cac1 transgene (Appl>cac1::GFP or elav>cac1::GFP). (B) Expression of the UAS-cacophony transgene lacking exon 7 in motor neurons using the D42-GAL4 driver was unable to rescue the crawling defects in cacexon7Δ mutants, whereas expression of the UAS-cacophony transgene containing exon 7 fully rescued crawling. Data represent the mean and s.e.m. of at least 10 animals and were analyzed using one-way ANOVA followed by Dunnett's multiple comparison test (F3,114=23.5; ***P<0.01, ****P<0.0001).