Fig. 4.

Inv^L/E:N^H cells exhibit ‘memory’ after prolonged exposure to a stiff niche. (A) Schematic of experimental design for cell commitment and migration assay. (B) Collective (left) and individual (right) migration was monitored for Inv^L/E:N^H cells on soft–stiff substrate combinations as indicated (soft=purple; stiff=orange). All data were analyzed using one-way ANOVA with Tukey's multiple comparisons test with *P<0.05, **P<0.01. n=23, 35, 61 and 58 cells for collective migration, and n=22, 27, 27 and 17 cells for individual migration from triplicate experiments.