Fig. 10.
Chronology of GC differentiation in Aldh1a1-3Germ−/−;Ser−/− testes at 7, 8, 9 and 10 days after synchronization. (A-D) Morphology of the spermatocytes, present at the center of the seminiferous tubules, assessed by Hematoxylin and Eosin staining. (E-L) Characterization of the spermatogonia populations, present at the periphery of the seminiferous tubules, assessed by detection of ZBTB16 (green signal) and either STRA8 or KIT (red signals), as indicated. (M,N) Expression of the meiotic markers SYCP3 and gH2AX (red signals) in leptotene spermatocytes. Nuclei were counterstained with DAPI (blue signal). Au, Aundiff spermatogonia; DPS, day post-synchronization; L, Z and P indicate leptotene zygotene and pachytene spermatocytes, respectively. Scale bar: 6 µm in A-D; 60 µm in E-L; 80 µm in M; 40 µm in N.