Fig. 3.
Synchronization of differentiation and Cre-mediated excision in spermatogonia. (A-F) Detection of STRA8 (red signal) and ZBTB16 (green nuclear signal) on histological sections from control, Aldh1a1-3Germ−/− and Aldh1a1-3Ser−/− testes 24 h after the administration of ATRA or sunflower oil at P3. (G-L) Detection of DDX4 (red cytoplasmic signal) and GFP (green membranous signal) on histological sections from Stra8-Cre;mT/mG and mT/mG mice 24 h after the administration of ATRA at P3. Efficient mT/mG excision by Stra8-Cre is assessed by GFP expression in almost all spermatogonia (G-I). In contrast, GFP is never detected in the absence of Stra8-Cre (J-L). The arrowheads in I indicate a few spermatogonia in which mTmG is not excised. Scale bar: 80 µm in A-F; 70 µm in G-L.