Fig. 8.
Inhibition of spermatogonia differentiation, but not meiosis, upon exposure of synchronized Aldh1a1-3Germ−/− testes to WIN18,446. (A) Pups were treated with ATRA at P3, with WIN18,446 or DMSO between 3 and 6 DPS, and their testes were analyzed at 7 DPS. (B,C) Spermatocytes expressing SYCP3 (red signal) and showing nuclear morphologies corresponding to zygotene spermatocytes (insets) fill the seminiferous tubules in both DMSO- and WIN18,446-treated pups. (D,E) STRA8-positive spermatogonia are present in DMSO-treated but totally absent in WIN18,446-treated pups (red signal at the periphery of the tubules). In contrast, STRA8 is expressed in zygotene spermatocytes in both DMSO- and WIN18,446-treated pups (red signal at the center of the tubules). (F,G) KIT-positive spermatogonia (red signal) are present in DMSO-treated but totally absent in WIN18,446-treated pups. Spermatogonia nuclei are stained using ZBTB16 (green signals), while all cell nuclei are stained using DAPI (blue signals). Au and Ad indicate Aundiff and Adiff spermatogonia, respectively. Z, zygotene spermatocytes. Scale bar: 60 µm.