Fig. 9.
Neonatal exposure of Aldh1a1-3Germ−/−;Ser−/− mutants to exogenous ATRA cannot sustain spermatogenesis. (A,B) Histological sections of testes from untreated 6-week-old mutant males. (C-F) Histological sections 6 weeks after a single injection of ATRA at P3. Genotypes are as indicated. All sections were stained with Hematoxylin and Eosin. E, elongated spermatids; G, spermatogonia; LY, Leydig cells; P, pachytene spermatocytes; R, round spermatids; SC, Sertoli cells; T and T*, tubule cross-sections displaying spermatogenesis or only Sertoli cells and spermatogonia, respectively. Scale bar: 60 µm in A-D; 20 µm in E,F.