Figure 5.

GSK2606414 exaggerated TUN effect on cell viability and salubrinal attenuated that. Cells were treated with PERK pathway inhibitors GSK or Sal at different concentrations for 1 hour before TUN treatment for 48 hours. Flow cytometry results show GSK reduced TM cell survival rate at all concentrations and reduced TMSC survival at higher concentrations (A). Sal increased cell survival rate in TM cells starting at 20 μM, whereas as low as 5 μM Sal protected TMSC from apoptosis (B). Results were normalized to DMSO controls and present as means ± SEM (n = 4). *Versus TUN without inhibitor treatment; ^#TMSCs versus TM cells with the same treatment. *^/#P < 0.05, **^/##P < 0.01, ***^/###P < 0.001. Two-way ANOVA followed by Dunnett's multiple comparison test.