FIGURE 2:

Intratetrad mating behavior following germination. (A) Genetically diverse wild strains used in this study. Image adapted from Strope et al. (2015). (B) Germinating tetrad spores from YJM1418 demonstrating intratetrad mating where all four spores mate (time after plating on rich media indicated in h:min). (C) Germinating tetrad spores from YJM1338 demonstrating all four spores entering cell cycle and producing buds rather than mating. (D) Quantification of mating behavior upon germination (n > 36 spores per strain). (E) Germinating tetrad spores from YJM1418 demonstrating one spore germinating and producing a bud prior to the germination of other spores in the same tetrad. Fourth panel shows the original early germinator mating with an intratetrad partner in its second cell cycle, as well as the other two spores mating. (F) Tetrad spores were separated from one another and then placed on an agarose slab containing glucose. Germination time was defined as the time between exposure to glucose and the first time point with a visible bud (n > 14 for each strain). (G, H) Relation between mating behavior from B and average germination time (G) or interspore variability in germination time (H) (assessed using the SD). Scale bar, 5 μm.