FIGURE 1:

RALY regulates PRMT1 expression, and ensuing FUS arginine methylation. (A) PRMT1 mRNA is enriched in RALY-containing RNPs. The RNA, purified after RALY or normal rabbit IgG IP in HeLa cell extract, was analyzed by qRT-PCR. The mRNA enrichment was calculated relatively to the 10% of RNA input. The scatter plot represents results from three independent experiments. The p value was calculated comparing PRMT1 with GAPDH using an unpaired two-tailed Student’s t test (*p < 0.05). (B) PRMT1 mRNA is down-regulated in RALY KO HeLa cells compared with control. PRMT1 mRNA was analyzed by qRT-PCR and normalized on GAPDH. The scatter plot represents results from three independent experiments. The p value was calculated with unpaired two-tailed Student’s t test (*p < 0.05). (C) PRMT1 protein is down-regulated in RALY KO HeLa cells compared with control cells. PRMT1 protein was analyzed by Western blot and normalized on ACTININ. The column graph represents means ± SEM results from five independent experiments. The p value was calculated with unpaired two-tailed Student’s t test (***p < 0.001). (D) FUS arginine methylation is decreased on RALY KO in HeLa cells. FUS protein was immunoprecipitated from RALY KO and control HeLa cells and analyzed by Western blot. The me-FUS band optical density was quantified and normalized on corresponding immunoprecipitated and input FUS bands. The scatter plot represents results from three independent experiments. The p value was calculated with unpaired two-tailed Student’s t test (*p < 0.05).