FIGURE 5:

RALY and FUS interact through their RNA-binding domains. (A) Western blot of protein extracts from HeLa cells, transfected with FUS-GFP constructs (Supplemental Figure S4), and probed with anti-GFP, anti-RALY, and anti-TUBULIN antibodies. (B) RALY coimmuno­precipitates with all FUS-GFP constructs but not with the one missing the RRM (Δ360-501). Co-IP experiment was performed by immunoprecipitating with anti-GFP antibody and probing the Western blot with anti-RALY antibody. (C–E) FUS-HA coimmunoprecipitates with all RALY-MYC/DDK constructs (Supplemental Figure S5) except the two missing the RRM (E). Co-IP experiment was performed by immunoprecipitating with anti-FLAG M2 affinity gel and probing the Western blots with anti-HA antibody and anti-Myc antibody. UT, untransfected; *, light IgG chain; **, heavy IgG chain.