FIGURE 7:
Nup2 is required to limit microtubule-dependent nuclear mobility after mitosis in early G1. Kymographs of NLS-DsRed during mitosis (yellow line) and for 30 min into G1 (red line) in (A) WT cells (SGS197) and (B) Δnup2 cells (SGS159-H). (C) Quantitation of total distance moved by nuclei in G1 analyzed for 15 min at 1 min intervals (n = 10 mitoses each for WT and Δnup2 cells. p value < 0.0001). (D) Kymographs of NLS-DsRed 30 min postmitosis followed by addition of 2.4 µg/ml benomyl for 15 min and after benomyl washout for 15 min.