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. 2019 Jan 14;9:3117. doi: 10.3389/fimmu.2018.03117

Figure 4.

Figure 4

Anti-proliferative and pro-apoptotic effects of IFN-γ are ameliorated in DFT1.Tet/IFN-γ cultured with doxycycline. Cell proliferation and viability of DFT1.Tet/IFN-γ was compared against DFT1.WT cells cultured with or without 50 ng/ml IFN-γ for 10 days. (A) Differences in proliferation of DFT1.Tet/IFN-γ in the presence and absence of doxycycline for 1, 2, 5, and 10 days, assessed by WST-8 proliferation assay. The WST-8 assay uses absorbance at 450 nm as a proxy for the number of viable cells. Baseline absorbance was measured 2 days prior (t = −2) and change in absorbance was measured after 2 days (t = 0). Graph shows the average value of triplicates and a standard deviation error bar. (B) Cell viability of DFT1.Tet/IFN-γ in the presence and absence of doxycycline for 1, 2, 5, and 10 days. Cell death over 2 days (from t = −2 to t = 0) was quantitated by staining dead cells with DAPI and analysis by flow cytometry. The results shown are representative of n = 3 replicates/treatment. The percentages in the upper right and left quadrants were added to yield the total percentage of dead cells while the same was done for the lower quadrants to give the total percentage of viable cells.