Genomic organization of PICV and tri-segmented rPICVtri. (A) Wild-type (WT) PICV genome consists of two genomic RNA segments (L and S), each encoding two viral genes in opposite orientation. (B) Tri-segmented recombinant PICV (rPICVtri) genome consists of three RNA segments, L, S1, and S2. S1 encodes GPC and the gene of interest (GOI-1). S2 encodes GOI-2 and NP. GOI, gene-of-interest; IGR, intergenic region.