Figure 5.

Altered Arc protein distribution during HSV-1 neuronal infection. Untreated (mock) or HSV-1-infected HT22 cells (MOI = 10) during 4, 8 or 18 hpi were stained with specific antibodies to Arc or to ICP8 followed by secondary antibodies coupled to Alexa Fluor 488 and 647 dyes, respectively. Phalloidin staining was coupled with Alexa Fluor 594 dye and Nuclei stained with DAPI. Scale bar corresponds to 10 μm. Magnification 630×. The results shown are representative of three independent experiments.