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. 2018 Dec 21;3(1):272–281. doi: 10.1089/can.2018.0065

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© Wesley M. Raup-Konsavage et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 1. — Cannabinoid receptors are expressed in CRC cell lines. RT-qPCR was performed to measure the levels of mRNA for each cannabinoid receptor in the seven CRC cell lines, and the control HEK 293 and CCD 814 CoTr cells (A) CNR1, (B) GPR55, and (C) TRPV1. Data are relative to GAPDH mRNA levels and are normalized to the expression in SW480 cells. As noted in the text, CNR2 RT-qPCR failed to produce reliable amplification curves (due to low or absent mRNA levels). Error bars are SEM. In general, HT-29 and LS174 cells expressed significantly higher levels of CNR1 and GPR55 mRNA (p≤0.05). CRC, colorectal cancer; mRNA, messenger RNA; RT-qPCR, reverse transcribed-quantitative polymerase chain reaction; SEM, standard error of the mean.