Fig. 7.

Evolution of transcription on the Pungitius pungitius Y chromosome. (A) Differences in expression between sexes are greater for genes in the P. pungitius SDR. At right is the ratio for the same genes in the threespine stickleback, where they are autosomal. (Significance tested with Mann–Whitney U tests.) To control for potential effect of mapping efficiency, M:F ratios for P. pungitius RNA-seq fold coverage were normalized by subtracting the M:F ratio calculated from DNA data. (B) Male:female expression ratios in brain tissue are predicted by Y:X differences within males. (D) Male:female expression ratios whole head tissue correlate with Y:X ratios within males, but less strongly than in brain tissue.