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. Author manuscript; available in PMC: 2019 Jan 21.
Published in final edited form as: Mol Immunol. 2010 May 11;47(11-12):2094–2102. doi: 10.1016/j.molimm.2010.04.002

Fig. 6.

Fig. 6

Foxp3 and SP3 but not SP1 repress p65-mediated ECR1_525 reporter activity. (A) Transient co-transfection of HEK 293 cells with 1 µg ECR1_525 luciferase reporter plasmid plus 1 µg p65 and increasing concentrations of SP1, SP3 or Foxp3 cDNAs. Luciferase activity was measured after 24 h. Bars represent triplicates; standard deviations are indicated. (B) Jurkat T cells were electroporated with 1 µg ECR1_525 pd2EGFP reporter and 1 µg pDsRed expression vector alone or in combination with 1 µg of the indicated transcription factor cDNAs. After electroporation the samples were cultured for 24 h in the presence or absence of 1 µg/ml platebound CD3 antibody OKT3. Bar graph shows the percentage of d2EGFP expressing cells among the transfected cells, which were identified and gated by DsRed expression. Bars represent triplicates; standard deviations are indicated.