Table 1. Transactivation of ECR1_525 by different transcription factors.

Transcription factor	A. Fold activation of luciferase reporter in HEK 293 cells	B. Percentage of d2EGFP expressing Jurkat T cells
		Unstimulated	PMA/iono
/	1.1 ± 0.2	1.1	2
AML1a	1 ± 0.2	1	2.7
AML1b	2.8 ± 0.3	1.2	1.9
EAPP	1.7 ± 0.1	1	2.5
E2F1	0.3 ± 0.1	1.4	1.8
E2F4	2.5 ± 0.4	0.9	2.2
EGR1	2.1 ± 0.5	1.4	2.7
Elk1	4.3 ± 0.1	0.7	1.5
Foxp3	1.6 ± 0.2	0.6	2
GATA1	4.7 ± 0.4	1.4	4.5
NFATc1a	1 ± 0.2	1.8	5.1
NFATc2/p	1.4 ± 0.4	1.2	3.1
PU.1	2.7 ± 0.3	2.5	3
p65	24.4 ± 3.9	3.5	10.9
cRel	1.6 ± 0.1	1.3	2.1
STAT1	0.6 ± 0.1	1.5	2.1
STAT5a	1.2 ± 0.4	2.3	4.1
SP1	1.3 ± 0	1,8	2.3
SP3	1.4 ± 0.4	3	1.5

(A) HEK 293 cells were transiently co-transfected with 1 µg ECR1_525 luciferase reporter plasmid alone or in combination with 2 µg of expression vectors encoding the indicated transcription factors. Luciferase expression was measured after 24 h. The data are the mean value ± SD of three independent experiments. (B) Jurkat T cells were electroporated using 2 µg ECR1_525 d2EGFP reporter plasmid in combination with 1 µg of either one of the indicated transcription factor cDNA or as control empty pCIneoHA vector. Samples were divided and treated for 22 h with or without PMA/ionomycin. Prior to flow cytometry measurement, the cells were treated with 1 µg/ml 7-AAD to distinguish dead cells from the living population.