(A) The levels of Rbm38, Pten, and actin transcripts were examined in the thumus from WT and Rbm38−/− mice. (B-C) The levels of Rbm38, Pten, and actin proteins were examined in Raji (B) or Romas (C) cells transiently transfected with scrambled or Rbm38 siRNA at 100 nM for 3 days. (D-F) The levels of Rbm38, Pten, and actin proteins were examined in isogenic control and Rbm38-KO U2OS (D), HCT116 (E), and Mia-Paca2 (F) cells. (G) The level of Rbm38, Pten and actin prpteins were examined in Mia-PaCa2 cells transfected with a control or Rbm38 expression vector for 24 h. (H-J) The levels of Pten and actin transcripts were examined by qRT-PCR in isogenic control and Rbm38-KO U2OS (H), HCT116 (I), and Mia-Paca2 (J) cells.