Figure 1.

PUF60 was overexpressed in breast cancer.

Notes: (A) Oncomine database was used to analyze PUF60 expression in patients with breast cancer or healthy individuals. (B, C) RT-PCR together with Western blotting technologies was recruited to determine the mRNA and protein expression of PUF60 in 30 matched breast cancer tissues and normal tissues (^**P<0.01). (D) The expression of PUF60 in 15 paired breast cancer tissues and normal tissues was detected by immunohistochemistry, and the score of PUF60 was recorded. (E, F) RT-PCR and Western blotting technologies were recruited to assess the mRNA and protein expression of PUF60 in breast normal MCF-10A cells and breast cancer MDA-MB-231, HCC1937, and MCF-7 cells. These experiments were repeated three times (^***P<0.001). (G) RT-PCR was used to evaluate the mRNA expression of PUF60 in the blood samples from 30 cases of breast cancer patients or 30 cases of healthy individuals.

Abbreviations: IHC, immunohistochemstry; RT-PCR, real-time PCR.