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. 2019 Jan 11;5(1):e000772. doi: 10.1136/rmdopen-2018-000772

Figure 2.

Figure 2

The secretome reveals multiple significantly upregulated cytokines in the salivary gland of patients with pSS. (A) Twenty-six cytokines were measured in the secretome of the validation cohort consisting of patients with nSS, iSS and pSS using Luminex. Cytokine expression data were quantile normalised around the median. Patients are sorted by subgroup: nSS (LFS 0 anti-SSAneg), iSS (anti-SSA+ and/or LFS>0) and pSS. The patients within the iSS and pSS groups are sorted by LFS (left to right: from low to high). Cytokines are sorted from low to high p value (from top to bottom) in pSS vs nSS, significantly differing cytokines are indicated in bold. (B) The secretome levels of CXCL13, CXCL10 and CCL19 correlate with LFS and with the mRNA expression of their respective gene as measured by RNA sequencing within the patients with pSS (Spearman R, p value). Cytokine concentrations below the detection limit were converted to the lowest point on the calibration curve (lower limit of quantification) multiplied by 0.5 (lower limit of detection). iSS, incomplete Sjögren’s syndrome; LFS, lymphocytic focus score; nSS, non-Sjögren’s sicca; pSS, primary Sjögren’s syndrome; SSA, Sjögren’s syndrome antigen A.