Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) |
E. coli BL21 star (DE3) | Thermo Fisher Scientific | C601003 | |
| Strain, strain background (Escherichia coli) |
E. coli DH5α (DE3) | Thermo Fisher Scientific | 18258012 | |
| Cell line (Spodoptera frugiperda) |
Sf9 | Thermo Fisher Scientific | 11496015 | |
| Peptide, recombinant protein |
Ccr4-Not | PMID: 27851962 | Purified recombinant protein expressed in Sf9 cells following infection with viruse produced from LP_Ccr4Not | |
| Peptide, recombinant protein |
Ccr4-Not (Ccr4-inactive) | PMID: 27851962 | Purified recombinant protein with Ccr4(E387) expressed in Sf9 cells following infection with virus produced from LP_P22-60 |
|
| Peptide, recombinant protein | Ccr4-Not (Caf1-inactive) |
PMID: 27851962 | Purified recombinant protein with Caf1(D53A) expressed in Sf9 cells followinginfection with virus produced from LP_P22-61 |
|
| Peptide, recombinant protein | MBP-Puf3 | this paper | Purified recombinant protein expressed inE. coli BL21star (DE3) following transformation with LP_P22-27 |
|
| Peptide, recombinant protein | MBP-Puf3(Δ63) | this paper | Purified recombinant protein expressed inE. coli BL21star (DE3) followingtransformation with LP_P22-28 | |
| Peptide, recombinant protein | MBP-Puf3(Δ125) | this paper | Purified recombinant protein expressed inE. coli BL21 star (DE3) following transformation with LP_P22-29 |
|
| Peptide, recombinant protein | MBP-Puf3(Δ181) | this paper | Purified recombinant protein expressed in E. coli BL2 1 star (DE3) following transformation with LP_P22-30 |
|
| Peptide, recombinant protein | MBP-Puf3(Δ240) | this paper | Purified recombinant protein expressed in E. coli BL21 star (DE3) following transformation with LP_P22-31 |
|
| Peptide, recombinant protein |
MBP-Puf3(Δ300) | this paper | Purified recombinant protein expressed in E. coli BL21star (DE3) following transformation with LP_P22-32 |
|
| Peptide, recombinant protein |
MBP-Puf3(Δ378) | this paper | Purified recombinant proteinexpressed inE. coli BL21 star (DE3) following transformation with LP_P22-33 | |
| Peptide, recombinant protein | MBP-Puf3(Mut) | this paper | Purified recombinant protein expressed inE. coli BL21star (DE3) following transformation with LP_P22-34 | |
| Peptide, recombinant protein | Puf3(PUM) | this paper | Purified recombinant protein expressed in E. coli BL21 star (DE3) following transformation with LP_P22-35 |
|
| Peptide, recombinant protein |
MBP-Zfs1(FL) | this paper | Purified recombinant proteinexpressed in E. coli BL21 star (DE3) following transformation with LP_P22-36 |
|
| Peptide, recombinant protein |
MBP-Zfs1(Δ34) | this paper | Purified recombinant protein expressed in E. coli BL21star (DE3) followingtransformation with LP_P22-37 | |
| Peptide, recombinant protein |
MBP-Zfs1(Δ71) | this paper | Purified recombinant protein expressed in E. coliBL21 star (DE3) following transformation with LP_P22-38 |
|
| Peptide, recombinant protein |
MBP-Zfs1(Δ182) | this paper | Purified recombinant protein expressed inE. coli BL21 star (DE3) followingtransformation with LP_P22-39 | |
| Peptide, recombinant protein |
MBP-Zfs1(Δ248) | this paper | Purified recombinant protein expressed in E. coli BL21 star (DE3) following transformation with LP_P22-40 |
|
| Peptide, recombinant protein |
MBP-Zfs1(Δ322) | this paper | Purified recombinant proteinexpressed in E. coli BL21 star (DE3) following transformation with LP_P22-41 | |
| Peptide, recombinant protein |
MBP-Zfs1(Mut) | this paper | Purified recombinant protein expressed in E. coli BL21 star (DE3) following transformation with LP_P22-42 |
|
| Peptide, recombinant protein |
Zfs1(TZF) | this paper | Purified recombinant protein expressed in E. coli BL21star (DE3) following transformation with LP_P22-43 |
|
| Peptide, recombinant protein |
Pab1 | PMID: 29932902 | ||
| Commercial assay or kit | Quikchange Lightning Multi Site-Directed Mutagenesis Kit |
Agilent Technologies | 210513 | |
| Commercial assay or kit | In-Fusion HD Cloning Kit | Takara Bio | 121416 | |
| Commercial assay or kit | Phusion High-Fidelity DNA Polymerase | New England BioLabs | M0530S | |
| Recombinant DNA reagent | MultiBac-Ccr4Not | PMID: 27851962 | LP_Ccr4Not | MultiBac expression vectorfor S. pombe Ccr4-Not |
| Recombinant DNA reagent | MultiBac-Ccr4Not(Ccr4 E387A) | PMID: 27851962 | LP_P22-60 | MultiBac expression vector forS. pombe Ccr4-Not (Ccr4 E387A) |
| Recombinant DNA reagent | MultiBac-Ccr4Not(Caf1 D53A) | PMID: 27851962 | LP_P22-61 | MultiBac expression vector forS. pombe Ccr4-Not (Caf1 D53A) |
| Recombinant DNA reagent | pMAL-c2x-Puf3(FL) | this paper | LP_P22-27 | pMAL-c2x expression vector forS. pombe Puf3(FL) (res1-734) |
| Recombinant DNA reagent | pMAL-c2x-Puf3(Δ63) | this paper | LP_P22-28 | pMAL-c2x expression vector for S. pombe Puf3(Δ63) (res63-734) |
| Recombinant DNA reagent |
pMAL-c2x-Puf3(Δ125) | this paper | LP_P22-29 | pMAL-c2x expression vector forS. pombe Puf3(Δ125) (res125-734) |
| Recombinant DNA reagent |
pMAL-c2x-Puf3(Δ181) | this paper | LP_P22-30 | pMAL-c2x expression vector forS. pombe Puf3(Δ181) (res181-734) |
| Recombinant DNA reagent |
pMAL-c2x-Puf3(Δ240) | this paper | LP_P22-31 | pMAL-c2x expression vector for S. pombe Puf3(Δ240) (res240-734) |
| Recombinant DNA reagent |
pMAL-c2x-Puf3(Δ300) | this paper | LP_P22-32 | pMAL-c2x expression vector for S. pombe Puf3(Δ300) (res300-734) |
| Recombinant DNA reagent |
pMAL-c2x-Puf3(Δ378) | this paper | LP_P22-33 | pMAL-c2x expression vector for S. pombe Puf3(Δ378) (res378-734) |
| Recombinant DNA reagent | pMAL-c2x-Puf3(Mut) | this paper | LP_P22-34 | pMAL-c2x expression vector for S. pombe Puf3(Mut) (res1-734; Y449A, Y671A) |
| Recombinant DNA reagent | pGEX6P-2-Puf3(PUM) | this paper | LP_P22-35 | pGEX6P-2 expression vector for S. pombe Puf3(PUM) (res378-714) |
| Recombinant DNA reagent | pMAL-c2x-Zfs1(FL) | this paper | LP_P22-36 | pMAL-c2x expression vector forS. pombe Zfs1(FL) (res1-404) |
| Recombinant DNA reagent | pMAL-c2x-Zfs1(Δ34) | this paper | LP_P22-37 | pMAL-c2x expression vector forS. pombe Zfs1(Δ34) (res34-404) |
| Recombinant DNA reagent |
pMAL-c2x-Zfs1(Δ71) | this paper | LP_P22-38 | pMAL-c2x expression vector forS. pombe Zfs1(Δ71) (res71-404) |
| Recombinant DNA reagent | pMAL-c2x-Zfs1(Δ182) | this paper | LP_P22-39 | pMAL-c2x expression vector for S. pombe Zfs1(Δ182) (res182-404) |
| Recombinant DNA reagent | pMAL-c2x- Zfs1(Δ248) |
this paper | LP_P22-40 | pMAL-c2x expression vector for S. pombe Zfs1(Δ248) (res248-404) |
| Recombinant DNA reagent | pMAL-c2x- Zfs1(Δ322) |
this paper | LP_P22-41 | pMAL-c2x expression vector for S. pombe Zfs1(Δ322) (res322-404) |
| Recombinant DNA reagent | pMAL-c2x-Zfs1(Mut) | this paper | LP_P22-42 | pMAL-c2x expression vector for S. pombe Zfs1(Mut) (res1-404; F349A, F387A) |
| Recombinant DNA reagent | pGEX6P-2-Zfs1(TZF) | this paper | LP_P22-43 | pGEX6P-2 expression vector forS. pombe Zfs1(TZF) (res699-769) |
| Recombinant DNA reagent | pGEX6P-2 -Pab1 |
PMID: 29932902 | LP_P22-11 | pGEX6P-2 expression vector forS. pombe Pab1 (res80-653) |
| Software, algorithm |
ImageJ | NIH | https://imagej.nih.gov/ij/ | |
| Software, algorithm |
GraphPad Prism 6 | GraphPad |
https://www.graph
pad.com/scientific- software/prism/ |
|
| Software, algorithm |
switchANALYSIS | Dynamic Biosensors |
https://www.dynamic-
biosensors. com/software/ |
|
| Software, algorithm |
ImageQuant | GE Healthcare | TL 5.2 | |
| Other | Insect-XPRESS protein-free insect cell medium withL-glutamine | Lonza | 12-730Q | |
| Other | Protease Inhibitor Cocktail | Sigma-Aldrich | 11836170001 | |
| Other | Desthiobiotin | IBA | 2-1000-001 | |
| Other | Imidazole | Sigma-Aldrich | I5513 | |
| Other | Formamide | Sigma-Aldrich | 11814320001 | |
| Other | TEMED | Sigma-Aldrich | T9281 | |
| Other | Ammonium persulfate (APS) | Sigma-Aldrich | A3678 | |
| Other | SYBR Safe DNA Gel Stain | Thermo Fisher Scientific | S33102 | |
| Other | SYBR Green II RNA Gel Stain | Thermo Fisher Scientific | S7586 | |
| Other | Ni-NTA Agarose | QIAGEN | 30210 | |
| Other | Glutathione Sepharose 4B | GE Healthcare | 17075601 | |
| Other | Amicon Ultra Centrifugal Filter Units | Millipore | UFC901096 | |
| Other | TWEEN 20 | Sigma-Aldrich | P9416 |