Table 1. Sequences of RNAs used in this study.
| Name | Sequence | Experiment |
|---|---|---|
| Zfs1 target A30 | AAUCAUCCUUAUUUAUUACCAUU[A]30 | Deadenylation |
| Puf3 target A30 / Puf3 target (5A) A30 |
AACUGUUCCUGUAAAUACGCCAG[A]30 | Deadenylation |
| Puf3 target (5U) A30 | AACUGUUCCUGUAUAUACGCCAG[A]30 | Deadenylation |
| Puf5 target | AACUGUUGUUGUAUGUACGCCAG[A]30 | Deadenylation |
| ARE/Zfs1 target | AUUAUUUAUU | Fluorescence polarization |
| PRE/Puf3 target (5A) | ACCUGUAAAUA | Fluorescence polarization |
| Puf3 target (5U) | ACCUGUAUAUA | Fluorescence polarization |
| Puf5 target | AGUUGUAUGUA | Fluorescence polarization |
| A30 | [A]30 | Fluorescence polarization |
| Puf3 target (5A) | ACCUGUAAAUAGGCG-[*DNA] | SwitchSENSE |
| Puf3 target (5U) | ACCUGUAUAUAGGCG-[*DNA] | SwitchSENSE |
| Puf5 target | AGUUGUAUGUAGGCG-[*DNA] | SwitchSENSE |
| Poly(A) | [A]15-[*DNA] | SwitchSENSE |
*DNA for hybridization to nanolever:.
ATCAGCGTTCGATGCTTCCGACTAATCAGCCATATCAGCTTACGACTA.