Table I.

Summary of Calculations for P-gp-specific and MDR Non-specific Efflux Indices using Mean Fluorescence Intensity (MFI) Values from Flow Cytometry of DiOC₂ and CD34+ Leukemic Blasts

Calculations Derived from MFI Values (DiOC2) of CD34+ Blast Cultures
1. P-gp Accumulation Ratio = Accumulation w/Zos, 37°C ÷ Accumulation w/o Zos, 37°C
2. P-gp Efflux Ratio = Efflux w/Zos, 37°C ÷ Efflux, 37°C
3. % Efflux (MDR Non-specific) = (Efflux, 4°C − Efflux, 37°C)*100 ÷ Efflux, 4°C
4. % Efflux w/Zos (MDR Non-specific) = (Efflux w/Zos, 37°C − Efflux,37°C)*100 ÷ Efflux w/Zos
5. % Inhibition of Efflux by Zos = (1-((Efflux, 4°C − Efflux, w/Zos, 37°C) ÷ (Efflux, 4°C − Efflux, 37°C)))*100