Table 2:

Each skin site is awarded a score between 0–5; a score of 0 reflects no evidence of depigmentation, and a score of 5 reflects 100% depigmentation at that site. Thus, the highest score each mouse can achieve is 20. For unbiased scoring of vitiligo mice, a blinded but trained investigator who is not aware of the experimental set up details should score the mice.

Vitiligo Scoring Point System
SCORE	0	1	2	3	4	5
TAIL	0	<10%	10–25%	>25–75%	>75–99%	100%
EAR	0	<10%	10–25%	>25–75%	>75–99%	100%
NOSE	0	<10%	10–25%	>25–75%	>75–99%	100%
FOOTPAD	0	<10%	10–25%	>25–75%	>75–99%	100%

Troubleshooting
Problem	Possible cause	Solution
High Pmel-1 CD8+ T cell number	Lymphoma in Pmel-1 donor	Use a different Pmel-1 donor with normal sized spleen. Transfer of Pmel-1 lymphoma cells may cause death of the recipient mouse
Poor or no induction of vitiligo	Poor injection technique	Practice retro orbital injections or ask a trained investigator for help with injections
Poor or no induction of vitiligo	Pmel-1 cells were killed	Investigate viability of CD8+ T cells after Miltenyi isolation and whole splenocytes after RBC lysis steps
Poor or no induction of vitiligo	Poorly mixed cells	Be sure to mix Pmel-1 cells before injection and do not leave cells in syringe for a long time
Poor or no induction of vitiligo	Poor viral titer	Re-titer viral stock
All the mice die	Mice were lethally irradiated	Be sure to calculate the time of radiation exposure so that mice receive sublethal dose of radiation (500 rads)