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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: DNA Repair (Amst). 2018 Aug 23;71:183–189. doi: 10.1016/j.dnarep.2018.08.023

Fig. 3. Strategies designed to study replication independent ICL repair employing laser localized tagged ICLs.

Fig. 3.

A. Laser-localized ICLs combined with immunofluorescence to study DDR activation by, and repair of, laser localized ICLs. γ-H2AX and the FAN1 nuclease appear in ICL stripes. Monitoring the Dig tag reveals repair of the ICL and the greater persistence of γ-H2AX.

B. Laser-localized ICL induction and proximity ligation assay (PLA) to distinguish DDR proteins that are proximal or distal to the tagged ICL.