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. 2018 Dec 28;9(102):37753–37765. doi: 10.18632/oncotarget.26514

Figure 2. ON108110 binds to the active site pocket of CK2.

Figure 2

(A) Unfolding of CK2α1 as monitored by DSF in the presence (apo protein) and absence of ON108110 (Figure 2a). (B) Dissociation constant of ON108110 in complex with CK2 as determined by MST. Binding curves were obtained using 16 concentrations of ON108110 (0.1 nM to 1,000nM). The Kd value was calculated using built-in curve fitting software (NanoTemper Technologies) and represents an average of 3 independent experiments. (C) 1.75 Å resolution structure of the CK2α1-ON 108110 co-crystal complex. ON 108110 (in green) occupies the ATP binding site of CK2α1. (D) ON108110 establishes hydrogen bonding with critical amino acids in the CK2α1 active site.