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. 2019 Jan 21;2:30. doi: 10.1038/s42003-019-0281-1

Fig. 2.

Fig. 2

Transcriptomic analysis of PpSnRK2 downstream factors. a Numbers of ABA-regulated genes and osmostress-regulated genes in wild-type (WT), and the numbers of genes affected in QKO plants. Genes with more than two-fold difference in the expression level between ABA-treated WT and QKO or mannitol-treated WT and QKO (false discovery rate (FDR) ≤ 0.05) were categorized as Decreased or Increased in QKO. b Principal component analysis of gene expression profile in QKO plants. c Fold-changes (FC) (log2) after 12 h of (left) 10 µM ABA treatment or (right) 0.4 M mannitol treatment of whole genes detected in the RNA-seq experiment were plotted. X-axis, WT; y-axis, QKO. d Hierarchical clustering and heatmap analysis were performed for the SnRK2-regulated ABA-inducible genes (SAGs). Gene expression values are represented as relative to the mean of all samples; blue represents lower expression, red represents higher expression (expression range, −3 to 3). Following high-throughput sequencing, data were obtained from the NCBI Sequence Read Archive, Auxin 5 h (SRR3350456), Dehydration 4 h (SRR1707403), Dehydration 12 h (SRR2225592), Heat stress (SRR790663), Salt 4 h (SRR1707400), and Young sporophyte (SRR1553314). e Gene ontology term enrichment analysis was performed against SAGs. Functional enrichment analysis was performed for BLAST best-hit genes of SAGs, which were used to search Arabidopsis TAIR 10 proteins using the BiNGO plug-in in Cytoscape. Gene ontology Slim was used to summarize the network. The intensity of the color indicates significance of nodes based on corrected p-value (5% FDR)