Figure 1.
Downregulation of let-7i expression in gastric cancer tissues and cell lines, effect of ectopic expression of let-7i on tumor cell viability, proliferative properties of SGC-7901, nude mouse xenograft formation and growth after restoration of let-7i expression. (A) qRT-PCR analysis of let-7i expression in 40 pairs of gastric cancer and their corresponding normal tissues. (B) Let-7i expression in gastric cell lines (SGC-7901, MGC-803, MKN-45, AGS, N87) compared with normal gastric epithelial cells (GES-1), as assessed on qRT-PCR. *P < 0.05 and **P < 0.01. (C) Cell viability assay for SGC-7901. *P < 0.05, **P < 0.01 and ***P < 0.001. (D) Colony formation assay for SGC-7901. *P < 0.05. (E) Wound healing assay for SGC-7901. *P < 0.05. (F) Transwell assay for SGC-7901 *P < 0.05. (G) Nude mouse xenograft assay. (H) Tumor weight formed by the indicated cells. ***P < 0.001. (I) Time-dependent tumor volumes (mm3) of miRNA negative control and let-7i mimics mice. *P < 0.05, **P < 0.01, and ***P < 0.001. (J) Pathologic sample and macroscopic observation in livers