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. 2018 Mar 19;10(2):120–130. doi: 10.1007/s13238-018-0526-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Assembly of D2 factors. (A–D) Structures of Rpf1 (A), Mak16 (B), Nsa1 (C) and Rrp1 (D). The N- and C-termini are labeled for each protein. The N-terminal domain (NTD) and middle domain (MD) of Mak16 are indicated. (E and F) Two views of Rpf1, Mak16, Nsa1 and Rrp1 assembled at the junction of domains I and II. AFs and individual domains of 25S rRNAs are color coded. L4, L18 and L32 are colored green, violet and yellow, respectively, and other RPLs are shown in gray. (G) Structure of mature 60S subunit (PDB code: 4V88) in the same view as (F)