Figure 1.

Replication competency of rescued recombinant measles virus (rMV). HEK293 cells stably expressing measles virus N, P, and T7 RNA polymerase were co-transfected with recombinant L plasmid (measles virus RNA dependent RNA polymerase) and measles virus full length genome, p(+)MV-NSE-FlagP-M502-3p (pMV) construct. Forty eight hours post transfection, cells were harvested and recombinant measles virus particles were rescued. To check the gene expression and the replication ability of recombinant virus recovered from packaging cell line; Vero and MCF-7 cells were infected with rescued virus and at 48 h post infection viral gene expression was checked. Viral gene expression in Vero and MCF-7 cells was checked for two successive passages and the images given here show the viral gene expression in the second passage. Expression of (A) N, P, and M transcripts by RT- PCR (B) N by IFA staining in Vero cells, (C) N, P, and M transcripts (D) N protein expression in MCF-7 cells.