Figure 4.

Combined effects of recombinant virus and H2 compound in breast cancer cells. MCF-7 and MDA-MB-231 cells were infected with rMV-BNiP3 followed by H2 compound treatment. Cells infected either with rMV or rMV-BNiP3 or treated with drug alone were used as controls. Infected cells were incubated at 37°C; CO₂ atmosphere and images of morphological changes induced were taken with microscope and compared with the respective controls. At 56 h post infection, cells were subjected to MTT assay to measure the percentage of cell viability. (A,C) Photomicrographs showing morphological changes induced following infection and drug treatment in MCF-7 and MDA-MB-231 cells, respectively. (B,D) Percentage of viability of infected MCF-7 and MDA-MB-231 cells treated with H2, respectively. ^*p ≤ 0.05; ^**p ≤ 0.01; ^***p ≤ 0.001 and ^****p ≤ 0.0001.