Figure 5.

Expression of PCNA as measurement of proliferative index. MCF-7 and MDA-MB-231 cells were grown on coverslips. At 80% confluency cells were infected with rMV-BNiP3 and treated with desired concentrations of paclitaxel or H2 compound. Cells were incubated at 37°C; 48 h post infection, cells were subjected to IFA staining with specific antibody to proliferative marker PCNA (proliferating cellular nuclear antigen). Nine different fields in each sample were analyzed by Image J software and the results were plotted in column graph with the mean values. (A) Expression of PCNA in MCF-7 and MDA-MB-231 cells, (B,C) Column graphs showing number of cells positive for nuclear antigen in MCF-7 and MDA-MB-231 cells, respectively. PCNA expression was significantly reduced in rMV-BNiP3 infected MDA-MB-231 cells treated with paclitaxel (p = 0.0326) or H2 (p = 0.051) as compared to cells treated with drugs alone. No significant effects were noted in MCF-7 cells. ^*p ≤ 0.05 and ^**p ≤ 0.01.