Figure 7.

Annexin V staining of MDA-MB-231 cells infected with recombinant virus and treated with drugs. MDA-MB-231 cells were infected with rMV or rMV-BNiP3 then treated with desired concentration of paclitaxel and H2 compound. Infected cells were harvested at 24 h post drug treatment and processed for FACS analysis using Alexa fluor 488 Annexin V/Dead cell apoptosis kit. A total number of 10,000 were analyzed and the data represented as column graph indicates percentage ratio of Annexin V/PI staining vs. Annexin V stainingin rMV-BNiP3 infected cells as compared to rMV infected cells or cells treated with drug alone. (A) Quadrants representing annexin V and annexin V/PI stained cells. Percentage of annexin V positive cells treated with (B) paclitaxel (C) H2 compound. Population of annexin V positive cells were higher in cells infected with rMV-BNiP3 (p = 0.0261) as compared to rMV. Cells treated with combination of rMV-BNiP3 with H2 (p = 0.0175) showed higher population of annexin V positive cells as compared to H2 alone. Cells infected with rMV-BNiP3 followed by treatment with paclitaxel showed higher population of annexin V positive cells as compared to its drug control (p = 0.0077) but showed significantly lower population of annexin V positive cells (p = 0.0004) as compared to the respective virus controls due to higher population of PI positive dead cells. ^*p ≤ 0.05; ^**p ≤ 0.01; ^***p ≤ 0.001 and ^****p ≤ 0.0001.