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Bdnf mRNA levels were measured by qPCR (A). BDNF level in the rat hippocampus lysate was detected by ELISA (B). The BDNF protein was detected by Western blot (C,D). Results were shown as mean ± s.d. (n=6). *P < 0.05 vs control group, #P < 0.05 vs AlCl3 group.
