Table 1. The influence of different OxPhos modulators on bioenergetic parameters.
Mito. Memb. Potential (CMXRos, potentiometric dye) | Oxygen Consumption Rate (Seahorse) | Reactive Oxygen Species (CellROX) | ATP (CellTiter-GLO) | |
---|---|---|---|---|
ETC blocker (rotenone) | ↓ | ↓ | ↑ | ↓ |
OxPhos uncoupler (FCCP) | ↓ | ↑ | ↓ | ↓ |
ATPase blocker (oligomycin) | ↑ | ↓ | ↑ | ↓ |
Substrate oxidation (succinate) | ↑ | ↑ | ↑ | ↑ |
Minerval | ↓ | ↑ | ↓ | ↓ |