Figure 2.

traC is expressed at high levels in the absence of TraM. Transcription of the traC promoter was assessed in both the presence and absence of TraM using a traC transcriptional fusion to lacZ on pRG970b::traC. This reporter plasmid was placed in trans to pAoF64/95ΔtraM in strain NTL4 harboring either pSRKGm or pSRKGm::traM. Both strains were grown in AB minimal medium with mannitol (light gray bars) or mannopine (dark gray bars) as the primary source of carbon and supplemented with 1 mmol/L IPTG to induce expression of traM. Samples of each culture were collected and assessed for β‐galactosidase activity as described in Experimental Procedures. n = 3 and error bars represent the standard deviation