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. 2019 Jan 21;9:245. doi: 10.1038/s41598-018-36475-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracellular ROS generation in human HCC cells induced by different concentrations of ADP extract (a) Photomicrographs showing intracellular ROS generation induced by two effective concentrations (15 and 25 mg/mL) of ADP extract after 12 h incubation. Photomicrographs were taken with a fluorescence microscope. Scale bar = 100 μm (b) The fluorescence in the cells is represented as the percentage of ROS production analyzed using flow cytometry.