Fig. 1. The presence of chlorobutanol in the formulation results in increased NAP concentration in the brain.

Experiments were performed as described in the Materials and Methods section (n = 3 per experimental group). a The upper panel shows the picture of two mice and the lower panel shows the Maestro results after the application of labeled NAP. b The upper panel shows the picture of two mouse brains and the lower panel shows the Maestro results, after the application of labeled NAP, decapitation and brain removal. c The presence of chlorobutanol in the formulation results in 4-fold more NAP in the brain. The Maestro results are shown after the application of labeled NAP, decapitation and brain removal. d Representative picture showing substantial Evans blue dye in peripheral organs. e Representative picture showing the intact brain of the animal presented in (d), showing no Evans blue dye penetration