Fig. 4.

Protein O-GlcNAcylation stabilizes the Treg cell lineage. a, b Flow cytometry of YFP⁺CD25⁺ cells among CD4⁺ T cells in the LNs (a) and frequencies of YFP⁺CD25⁺ cells in the LNs and spleen from 2-week-old Foxp3^YFP-Cre/Y Ogt^wt/Y and Foxp3^YFP-Cre/Y Ogt^fl/Y mice, n = 8 each group (b). c, d Flow cytometry of FOXP3⁺CD25⁺ cells among CD4⁺ T cells (c) and MFI of FOXP3 in FOXP3⁺CD25⁺ Treg cells (d, n = 3–4) in the LNs from 2-week-old Foxp3^YFP-Cre/Y Ogt^wt/Y and Foxp3^YFP-Cre/Y Ogt^fl/Y mice. e MFI of FOXP3 in OGT-sufficient and -deficient Treg cells in the LNs from Foxp3^YFP-Cre/Y Ogt^fl/fl mice, n = 5. f Histogram of FOXP3 expression in CD4⁺CD25⁺YFP⁺ Treg cells in the LNs from 2-week-old Foxp3^YFP-Cre/Y Ogt^wt/Y and Foxp3^YFP-Cre/Y Ogt^fl/Y mice, n = 6 each group. g Flow cytometry and quantification of the frequencies of Td-tomato⁺GITR⁺FOXP3⁻ ex-Treg cells among CD4⁺ T cells in the LNs from Foxp3^{eGFP-Cre-ERT2/Y} Ogt^wt/Y Rosa26^tdTomato/wt and Foxp3 ^{eGFP-Cre-ERT2/Y} Ogt^fl/Y Rosa26^tdTomato/wt mice, n = 4. h, i T-BET⁺, GATA3⁺ and RORγT⁺ cells among ex-Treg cells in the LNs from Foxp3^{eGFP-Cre-ERT2/Y} Ogt^wt/Y Rosa26^tdTomato/wt and Foxp3 ^{eGFP-Cre-ERT2/Y} Ogt^fl/Y Rosa26^tdTomato/wt mice, n = 3–4. Data are shown as mean ± s.e.m. *p < 0.05; **p < 0.01; ***p < 0.001 by unpaired (b, d, g–i) and paired (e) student’s t-test