Fig. 5.

O-GlcNAcylation is required for the effector differentiation of Treg cells. a–d Frequencies of CD44^hiCD62L^lo (a), CD44⁺KLRG1⁺ (b), CD44⁺PD-1⁺ (c), and CD44⁺CD73⁺ (d) cells among YFP⁺CD25⁺ Treg cells in the LNs and spleen from 2-week-old Foxp3^YFP-Cre/Y Ogt^wt/Y and Foxp3^YFP-Cre/Y Ogt^fl/Y mice, at least n = 4 each group. e–i Frequencies of indicated cell populations among YFP⁻OGT-sufficient and YFP⁺ OGT-deficient CD4⁺TCRβ⁺CD25⁺GITR⁺ Treg cells in the LNs and spleen from Foxp3^YFP-Cre/wt Ogt^fl/fl mice, n = 3 each group. Data are shown as mean ± s.e.m. *p < 0.05; **p < 0.01; ***p < 0.001 by unpaired student’s t-test