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. 2019 Jan 21;10:354. doi: 10.1038/s41467-019-08300-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Attenuated IL-2/STAT5 signaling in OGT-deficient Treg cells. a Hierarchical clustering of top differentially expressed genes (60 genes with FDR q values less than 0.1) between OGT-sufficient and OGT-deficient Treg cells using Morpheus. b, c Enrichment plots for up-regulated (b) and down-regulated (c) genes in Blimp1⁺ eTreg cells from the Gene Set Enrichment Analysis (GSEA) of differentially expressed genes between OGT-sufficient and OGT-deficient Treg cells. d Ingenuity Pathway Analysis (IPA) of predicted upstream regulators for observed changes in gene expression. e–j Foxp3^YFP-Cre/wt Ogf^fl/fl female mice were injected with PBS or the IL-2 immune complex (n = 2–5) for 3 consecutive days and Treg cells were analyzed at day 6. Flow cytometry of CD44⁺KLRG1⁺(e), CD4⁺GZMB⁺ (f) and CD4⁺BLIMP-1⁺ (g) Treg cells among YFP⁻OGT-sufficient and YFP⁺ OGT-deficient Treg cells (CD4⁺CD25⁺GITR⁺) in LNs and spleen and corresponding frequencies in h–j. k Expression of STAT5-target genes in OGT-sufficient and OGT-deficient Treg cells. l–m mRNA levels of Socs1 (l) and Socs3 (m) in OGT-sufficient Treg cells after retrovirus infection as indicated, n = 3 each group. Data are shown as mean ± s.e.m. *p < 0.05; **p < 0.01; ***p < 0.001 by two-way ANOVA (h–j) and one-way ANOVA (l, m)