Fig. 4.

Acute stress occludes photoactivation of the DRN 5-HT circuit. a Experiment schematic. b Second level analysis comparing restraint (n = 7) to control (n = 4) condition indicate a decrease in negative response amplitude in the restraint group relative to control across the brain. Colour scale = t-statistic (non-parametric test, p ≤ 0.05, cluster corrected), blue indicating significance for restraint > control comparison; see Supplementary Figure 13 for all brain slices. c COPEs comparing restraint (blue) and control (red) indicate reduced response amplitudes in the majority of responding ROIs, including DRN (*p < 0.05, FDR corrected). Bound of box and centre line represent 25th, 50th, 75th percentiles, whiskers represent 1.5*inter-quartile range. d The blunted response to photoactivation following acute stress persists across the three consecutive scans of each session. Error bars indicate ±1 standard deviation