Fig. 3. Cyfip1 haploinsufficiency leads to decreased stability of dendritic spines without affecting their formation.

a Dendrites of GFP-expressing neurons from the motor cortex (M1), visual cortex (V1), and hippocampus (CA1 and CA3) of Cyfip1^WT and Cyfip1^HET mice. Scale bars represent 10 μm. Quantification of spine density of Cyfip1^WT and Cyfip1^HET dendrites. b Two images of the same dendrite of a Cyfip1^WT mice motor neuron taken at an interval of 2 days showing the newly formed dendritic spines (arrows). Scale bars represent 2 μm. c Quantification of formed and eliminated dendritic spines in motor cortices of Cyfip1^WT and Cyfip1^HET mice. d Quantification of formed dendritic spines in motor cortices of Cyfip1^WT and Cyfip1^HET mice before (B) and after rotarod training (R). All values are represented as mean ± SEM. In (b) and (d), statistical significance was tested by ANOVA followed by Bonferroni post-hoc test. **P ≤ 0.01