Fig. 6.

BAP1 function relies on its deubiquitinase activity against H2AK119ub1. a Reintroduction of wild-type or catalytically dead (C91S) BAP1 in BAP1 KO cells. Top panel: western blot analysis of BAP1 expression in cytoplasmic (C) or nuclear (N) fractions. HDAC1 is used as a loading control. Bottom panel: RT-qPCR analysis of three BAP1-regulated genes in wild-type, BAP1 KO and the two rescue conditions. Horizontal bars indicate the mean expression. n = 3. b Western blot analysis of RING1A, RING1B, BAP1, H2AK119ub1, and H3K27me3 in wild-type, RING1A/B double KO, or RING1A/B; BAP1 triple KO HAP1 cells. H4 is used as a loading control. c Venn diagram showing the overlap between genes downregulated in BAP1 KO or upregulated in RING1A/B dKO cells. d Box-plots (median, lower, and upper quartiles, lowest and highest values) of log2 transcript per million (TPM) expression values of BAP1-only-regulated genes (top) and BAP1/PRC1-regulated genes⁶¹ in WT and RING1A/B dKO cells. P-value of the Mann–Whitney test on WT versus RING1A/B dKO comparison is indicated. e Scatterplot showing log2 fold-change (logFC) expression between RING1A/B dKO and RING1A/B; BAP1 tKO cells as a function of average log2 counts per million (logCPM). BAP1, the only differentially expressed genes is highlighted in purple. f RT-qPCR analysis of CYP26A1, RARB, DHRS3, and FAM46A expression following RA treatment at different time-points in wild-type or BAP1, RING1A/B, RING1A/B;BAP1 KO cells