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. 2019 Jan 21;10:360. doi: 10.1038/s41467-018-08126-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Enhancer and promoter profiles in smFISH. a, b Proportion of cells with KLF6-eRNA1 and PMEPA1-eRNA1 detected by a FISH, b C1 CAGE. c Proportion of cells with detected gene intronic RNA and eRNA and cells with spot overlap at the KLF6 and PMEPA1 loci. d Representative images showing gene intronic RNA and eRNA detection by FISH. Bar = 5 μm. n = 100 per time point. Source data are provided as a Source Data file